This facility was established by a grant from
Health Resources and Services Administration (HRSA)

Charles E. Schmidt College of Science

Division of Research & Graduate Studies

Catalog Affymetrix GeneChip Expression Arrays / NetAffx Analysis Center / Basis for Technology / Useful Links / We Welcome Your Comments / Questionnaire / Additional Services
Rapid Cycle Real-Time (kinetic) PCR using LightCycler instumentation (Roche)

Introduction / Quantitative RT-PCR / PCR Primer Design / Sample preparation / Amplification
Data Analysis / References / Services and Fees / RT-PCR Services Request Form

Services and Fees

Services

 

FAU Investigators

 

External Investigators

 

RNA isolation (sample)

 

$50.00

 

$100.00

 

Sample preparation (sample)

 

$10.00

 

$20.00

 

RT-PCR Set-up Fee (1 time fee)
1 time fee per reference gene set.

 

$20.00

 

$40.00

 

Standard curve preparation (1 time fee)
Includes choice of housekeeping gene set.
1 time fee per reference gene set.
(Standards are run in duplicate)

 

$60.00

 

$120.00

 

Quantitative RT-PCR (sample)
Includes choice of housekeeping gene reference
primers, target gene primers and data analysis.
(Samples are run in duplicate)

 

$15.00

 

$30.00

 

Primer design
LightCycler Probe Design Software
is used with investigator supplied template
sequence stripped of all annotations.

 

$10.00

 

$20.00

 

Primer synthesis

 

$0.40 / base

 

$0.80 / base

 
Plus shipping and handling
 

Note:

Primers may be supplied by the investigator as HPLC or gel purified oligonucleotides at a concentration of 25 uM in nuclease free water (minimun of 10 ul). If you need information on oligonucleotide suppliers, please contact us.

Total RNA may be supplied by the investigator with the following requirements:

5 ug / sample

Minimun concentration of 0.25ug/ul

An A260/A280 ratio between 1.8-2.0

Gel image or equivalent