Edward F. Orlando PhD: University of Florida (2001) Assistant Professor Contact information: Florida Atlantic University Department of Biological Sciences Harbor Branch Oceanographic Institution 5775 N. Old Dixie Hwy, Ft. Pierce, FL34946 Phone: ( 772) 462-0888 Email: eorlando@fau.edu

Research interests:
My lab integrates molecular, endocrinological, and morphological endpoints to investigate the environment – genome interaction and its effect on development and reproduction. Through a combination of lab and field studies, my students and I study how environmental factors affect the (1) development of sex specific characteristics and (2) brain – pituitary – gonadal axis regulation of reproduction in fishes.

 Publications (Student co-authors are shown in bold with an asterisk)

1. EF Orlando, DE Bass* LM Caltabiano*, WP Davis, LE Gray, Jr. and LJ Guillette, Jr.  Altered development and reproduction in mosquitofish exposed to paper mill effluent in the Fenholloway River, Florida USA.  Aquatic Toxicology 84(4): 399 - 405 (2007).
2. K Watanabe, K Jensen, EF Orlando, and GT Ankley.  What is Normal?  A characterization of the values and variability in apical reproductive parameter data of the fathead minnow, Pimephales promelas. Comparative Biochemistry and Physiology 146(3): 348-356 (2007).
3. EF Orlando, GA Binczik, ND Denslow, and LJ Guillette, Jr. Reproductive seasonality of the female Florida Gar, Lepisosteus platyrhincus. General and Comparative Endocrinology 151(3):318-324 (2007).
4. J Burger, C Fossi, P McClellan-Green, and EF Orlando. Methodologies, bioindicators, and biomarkers for assessing gender-related differences in wildlife exposed to environmental chemicals. Environmental Research 104(1):135-152 (2007).
5. EF Orlando and LJ Guillette, Jr. Sexual dimorphic responses in wildlife exposed to endocrine disrupting chemicals. Environmental Research 104(1):163-173(2007).
6. A Kanamori, A Yamamura, S Koshiba, J-S Lee, EF Orlando, and H. Hori. Methyltestosterone efficiently induces male development in the self-fertilizing hermaphrodite fish, Kryptolebias marmoratus. Genesis: the Journal of Genetics and Development 44: 495 - 503 (2006).
7. EF Orlando, Y Katsu, S Miyagawa, and T. Iguchi. Cloning and differential expression of the estrogen receptor and aromatase genes in the self-fertilizing hermaphrodite and male mangrove rivulus, Kryptolebias marmoratus. Journal of Molecular Endocrinology 37: 353 - 365 (2006).
8. EF Orlando, GA Binczik, J Gates*, LE Gray Jr., M Horton*, AS Kolok, C Lambright, and LJ Guillette, Jr. Endocrine disrupting effects of cattle feedlot effluent on a sentinel species, the fathead minnow. Environmental Health Perspectives 112: 353-358 (2004).
9. AM Soto, JM Calabro*, NV Prechtl, AY Yau, EF Orlando, A Daxenberger, AS Kolok, LJ Guillette, Jr., BL Bizec, IG Lange, and C Sonnenschein. Androgenic and estrogenic activity of cattle feedlot effluent receiving water bodies of eastern Nebraska, USA. Environmental Health Perspectives 112: 346-352 (2004).
10. EF Orlando, GA Binczik, L Ford, P Thomas, and LJ Guillette, Jr. Reproductive seasonality of the male Florida Gar (Lepisosteus platyrhincus). General and Comparative Endocrinology 131(3) 365-371 (2003).
11. EJ Durhan, C Lambright, V Wilson, BC Butterworth, DW Kuehl, EF Orlando, LJ Guillette, Jr., LE Gray, Jr., and GT Ankley. Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluent. Environmental Toxicology and Chemistry 21(9):1973-1976 (2002).
12. EF Orlando, WP Davis, LJ Guillette, Jr. Aromatase activity in the ovary and brain of the mosquitofish, Gambusia holbrooki, exposed to paper mill effluent. Environmental Health Perspectives 110 (Supplement 3) 429-433 (2002).

     For a complete publication list and other information, please see my C.V.

Current Funding

USEPA - STAR Grant. Computational Toxicology and Endocrine Disruptors Program. Systems biology modeling of the fathead minnow and zebrafish responses to neuroendocrine active chemicals.Co-PI with Drs. G Ankley, D Bencic, T Collette, N Denslow, M Sepúlveda, and K Watanabe.

Environmental Health Science Communication Fellowship (February 2007 – January 2008, $5,000).