Herbert Weissbach, Ph.D. Distinguished Research Professor and Director, Charles E. Schmidt College of Science Center for Molecular Biology & Biotechnology Florida Atlantic University 777 Glades Road Sanson Science, Room 220 Boca Raton, FL 33431 561) 297-2596 (office) (561) 297-2597 (laboratory) Email: hweissba@fau.edu

Education

• 1957 Ph.D. George Washington University (Thesis: Studies on 5-Hydroxyindole Metabolism)

Recent Publications

• Ciorba,M.A., Heinemann,S. H., Weissbach H., Brot N., Hoshi T. Modulation of Potassium Channel Function by Methionine Oxidation and reduction. Proc. Natl. Acad. Sci. USA (1997) 94,9932-9937.
• Weissbach, H. A Revisit to Bacterial Protein Synthesis: The Search for the Role of GTP. Protein Science Journal, (1998) 7, 516-521.
• Ciorba, M. A., Heinemann, S. H., Weissbach, H., Brot, N., and Hoshi, T. Regulation of voltage-dependent K+ channels by methionine oxidation: effect of nitric oxide and vitamin C. FEBS Letters, (1999) 442, 48-52.
• Kuschel, L., Hansel, A., Schonherr, R., Weissbach, H., Brot, N., Hoshi, T., and Heinemann, S. H. Molecular cloning and functional expression of a human peptide methionine sulfoxide reductase (hMsrA). FEBS Letters, (1999) 456, 17-21.
• Yermolaieva, O., Brot, N., Weissbach, H., Heinemann, S., and Hoshi, T. Reactive Oxygen Species and Nitric Oxide Mediate Plasticity of Neuronal Calcium Signaling. Proc. Natl. Acad. Sci. USA. (2000) 97, 448-453.
• Lowther, W. T., Brot, N., Weissbach, H., Honek, J., and Matthews, B. Thiol-disulfide exchange is involved in the catalytic mechanism of peptide methionine sulfoxide reductase. Proc. Natl. Acad. Sci. USA. (2000) 97, 6463-6468.
• Brot, N., and Weissbach, H. Peptide methionine sulfoxide reductase: Biochemistry and Physiological Role. Biopolymers and Peptide Science. (2000) 55, 288-296.
• Lowther, W. T., Brot, N., Weissbach, H., and Matthews, B. W. Structure and mechanism of methionine sulfoxide reductase, an “anti-oxidation” enzyme. Biochemistry. (2000) 39, 13307-13312.
• Tang, X. D., Daggett H., Hanner, M., Garcia M. L., McManus O. B., Brot N., Weissbach, H., Heinemann S. H., Hoshi T. Oxidative Regulation of Large Conductance Calcium-activated Potassium Channels. JGen Physiol. (2001) 117(3), 253-274.
• St John, G., Brot, N., Ruan, J., Erdjument-Bromage, H., Tempst, P., Weissbach, H., Nathan, C. Peptide Methionine Sulfoxide Reductase from Escherichia coli and Mycobacterium tuberculosis Protects Bacteria Against Oxidative Damage from Reactive Nitrogen Intermediates. Proc. Natl. Acad. Sci. USA. (2001) 9 8, 9901-9906.
• Forster, A. C., Weissbach, H., and Blacklow, S. C. A reconstruction of Oligopeptide Synthesis From Purified E. coli Components Using Five His-Tagged Initiation and Elongation Factors and the Epsilon Enhancer Sequence. Analytical Biochemistry. (2001) 297(1), 60-70.
• Weissbach, H., Etienne, F., Hoshi, T., Heinemann, S. H., Lowther, W. T., Matthews, B., St. John , G., Nathan, N., Brot, N. Peptide Methionine Sulfoxide Reductase: Structure, Mechanism of Action, and Biological Function. Arch. Biochem. Biophys. (2002) 397(2), 172-178.
• Ruan, H., Tang, X. D., Chen, M. -L., Joiner, M.A., Sun, G., Brot, N., Weissbach, H., Heinemann, S. H., Iverson, L., Wu, C. -F., Hoshi, T. High-quality life extension by the enzyme peptide methionine sulfoxide reductase. Proc. Natl. Acad. Sci. USA. (2002) 99, 2748-2753.
• Lowther, W. T., Weissbach, H., Etienne, F., Brot, N., Matthews, B. W. The “Mirrored” Methionine Sulfoxide Reductases of Neisseria gonorrhoeae pilB. Nature Structural Biology . (2002), 9 (5), 348-352.
• Skaar, E. P., Tobiason, D. M., Quick, J., Judd, R. C., Weissbach, H., Etienne F., Brot, N., Seifert, H. S. The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species. Proc. Natl. Acad. Sci. USA. (2002), 99, 10108-10113
• Etienne, F., Spector, D., Brot, N., Weissbach, H. A methionine sulfoxide reductase in Escherichia coli that reduces the R enantiomer of methionine sulfoxide. Biochem. & Biophys. Res. Comm. (2003), 300, 378-382.
• Spector, D., Etienne, F., Brot, N., Weissbach, H. New Membrane Associated and Soluble Peptide Methionine Sulfoxide Reductases in Escherichia coli . Biochem. & Biophys. Res. Comm. (2003), 302, 284-289.
• Weissbach, H., Witkop, B. Biographical Memoirs: Sydney Udenfriend. PNAS Biographical Memoirs, Volume 83 (2003)
• Etienne, F., Resnick, L., Sagher, D., Brot, N., Weissbach, H. Reduction of Sulindac to its Active Metabolite, Sulindac Sulfide: Assay and Role of the Methionine Sulfoxide Reductase System. Biochem. & Biophys. Res. Comm. (2003), 312, 1005-1010.
• Yermolaieva, O., Xu, R., Schinstock, C., Brot, N., Weissbach, H., Heinemann, S., and Hoshi, T. Methionine Sulfoxide Reductase A (MSRA) Protects Neuronal Cells Against Brief Hypoxia-Reoxygenation. Proc. Natl. Acad. Sci. USA. (2004), 101, 1159-1164.
• Weissbach, H., Resnick, L., Brot, N. Methionine Sulfoxide Reductases: History and Cellular Role in Protecting Against Oxidative Damage. Biochimica & Biophysica Acta. (2005), 1703, 203-212.
• Marchetti, M., Pizarro, G. O., Sagher, D., DeAmicis, C., Brot, N., Hejtmancik, J. F., Weissbach, H., Kantorow, M. Methionine sulfoxide reductases B1, B2 and B3 are present in the human lens and confer oxidative stress resistance to lens cells. Investigative Ophthalmology and Visual Science. (2005), 46, 2107-2112.
• Lee, J., Gordiyenko, N., Marchetti, M., Tserentsoodol, N., Sagher, D., Alam, S., Weissbach, H., Kantorow, M., Rodriguez, I. Gene structure, localization and role in oxidative stress of methionine sulfoxide reductase A (msrA) in the monkey retina. Journal of Experimental Eye Research. (2005) (In Press).